《植物生理学报》 2015, 51(7): 1133-1141

蓝莓花芽休眠与解除过程中生理生化变化及DNA甲基化差异分析

李波, 夏秀英^*, 刘思

大连理工大学生命科学与技术学院, 辽宁大连116024

通信作者：夏秀英；E-mail: xx47@dlut.edu.cn；Tel: 0411-84706356

摘　要：

以‘伯克利’蓝莓花芽为材料, 研究了其在内休眠及解除休眠过程中部分生理生化指标的变化, 并对内休眠与解除休眠花芽进行了基因组DNA甲基化敏感扩增多态性(methylation sensitive amplified polymorphism, MSAP)分析。结果显示, 蓝莓花芽中脯氨酸、可溶性糖、可溶性蛋白含量及SOD、POD活性的变化趋势与休眠进程密切相关。内休眠期间, 脯氨酸含量呈先降后升的趋势, 可溶性糖含量及POD活性持续上升, 可溶性蛋白含量及SOD活性持续下降; 随着休眠开始解除, 可溶性糖、可溶性蛋白含量及SOD活性均上升, 而POD活性下降。MSAP分析表明, 蓝莓花芽内休眠期间, DNA甲基化水平较高, 并以全甲基化为主; 内休眠花芽总甲基化率(38.41%)、全甲基化率(27.09%)及半甲基化率(11.32%)均明显高于解除休眠花芽; 相对于内休眠花芽, 解除休眠花芽约84.48%的位点发生了甲基化模式变化, 其中去甲基化位点数和比例分别为248和40.52%, 超甲基化的位点数和比例分别为185和30.23%。DNA甲基化参与了蓝莓内休眠的调控。

关键词：蓝莓; 休眠; 生理生化特性; DNA甲基化; 甲基化敏感扩增多态性

收稿：2015-04-13   修定：2015-06-04

资助：辽宁省农业科技创新团队项目(2014204007)。

Changes in Physiological and Biochemical Properties and Variation in DNA Methylation Patterns during Dormancy and Dormancy Release in Blueberry (Vaccinium corymbosum L.)

LI Bo, XIA Xiu-Ying^*, LIU Si

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China

Corresponding author: XIA Xiu-Ying; E-mail: xx47@dlut.edu.cn; Tel: 0411-84706356

Abstract:

Changes in soluble sugar and protein contents, superoxide dismutase (SOD) and peroxidase (POD) activities in floral buds of ‘Berkeley’ were studied during dormancy period. The results indicated that the proline content showed a rising trend after an initial drop, the soluble sugar content and the POD activity continued to rise, the soluble protein content and SOD activity continued to decline during endodormancy. With the beginning of dormancy release, the soluble sugar and protein contents, the SOD activity increased, while the POD activity decreased. DNA methylation patterns were subsequently analyzed by methylation sensitive amplified polymorphism (MSAP) technology with 56 pairs of primer. The results showed that the level of methylation in dormant buds was high, among which full methylation was dominant. The levels of total DNA methylation, hemi-methylation and fully methylation were down-regulated during bud dormancy release. About 84.48% methylation loci varied during dormancy release when compared with dormancy, among which DNA demethylation loci were 248 with percentages of 40.52%, DNA hypermethylation loci were 185 with percentages of 30.23%. The results implied that DNA methylation is involved in the regulation of the dormancy in blueberries.

Key words: blueberry; dormancy; physiological and biochemical characteristics; DNA methylation; methylation sensitive amplified polymorphism